In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer

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In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer.

SCID patients have been successfully treated by administration of ex vivo gene-corrected stem cells. However, despite its proven efficacy, such treatment carries specific risks and difficulties. We hypothesized that some of these drawbacks may be overcome by in situ gene correction of T lymphoid progenitors in the thymus. Indeed, in vivo intrathymic transfer of a gene that provides a selective ...

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Viral and non-viral vectors have been developed for gene therapy, but their use is associated with unresolved problems of efficacy and safety. Efficient and safe methods of DNA delivery need to be found for medical application. Here we report a new monopolar system of non-viral electro-gene transfer into the thymus in vivo that consists of the local application of electrical pulses after the in...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 2005

ISSN: 0021-9738

DOI: 10.1172/jci23966